Temperature programming: optimal performance finally available in Micro GC.
Applying a temperature ramp during chromatographic analysis allows optimization of analytical parameters to the set of compounds being detected.
Thus, in a complex mixture and for a given column, some compounds require a low column temperature to ensure their separations; while others require a higher temperature to guarantee the most punctual elution possible and ensure a fine peak by maximizing its signal to noise ratio.
In isothermal, the user must resolve to determine a temperature that represents a compromise between the lightest and heaviest compounds. See it may be necessary to add an additional module to cover the whole range of compounds.
Thanks to the temperature programming, we now have optimal conditions for the entire working range of the column.
Temperature programming results in improved performance :
un sensitivity gain for the compounds eluted at the end of the chromatogram by a conservation of the chromatographic performances
Improved peak resolution that results in simplified integration and makes its operation much more robust over time
Another benefit of temperature programming is the time stability of the analyzer. This is true and notable primarily for the Molecular Sieve and PLOT columns. These columns, adapted for the separation of the smallest molecular species such as permanent gases (H2, O2, N2, CO, CH4, CO2), are sensitive to humidity (H2O) and heavier species (hydrocarbons).
Over time, in isothermal conditions, the accumulation of heavy species or moisture loads the column and the retention times change. As a result, the stability of the analyzer over time is compromised.
Temperature programming overcomes this limitation. Indeed, by applying temperature programming to each analysis, moisture and heavy species are eluted at each run and therefore do not accumulate in the column. The micro GC Fusion therefore ensures stable results over time.
Keywords: micro gc fusion, temperature programming, biomethane, permanent gas